Development of a Simple and Low-Resource Regeneration System of Two Greek Tomato Varieties

Native tomato landrace varieties (Solanum lycopersicum L.) are sources of high genetic diversity and adaptable to local environmental conditions. The in vitro propagation system can be used as a tool produce disease-free, high-quality material preserve the unique characteristics commercial Greek varieties, such variety Areti traditional Makedonia, both exceptional quality economic importance. Twenty- 27-day-old cotyledon leaf explants were cultivated 4 regeneration media supplemented with 0 or 0.1 mg∙L−1 indole-3-acetic acid 0.5 1 zeatin. Regeneration efficiency was dependent on variety, explant type age, media. more appropriate target material, exhibiting six-fold greater response compared regardless age. regenerated shoots successfully rooted (60%) acclimatized (98%). This protocol would valuable production for conservation practices breeding programs improvement.